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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Cell Biol. 2009 Aug 9;11(9):1121–1127. doi: 10.1038/ncb1926

Figure 5. Boz depletion mitigates dorsalization in lnx-l morphants.

Figure 5

a–d, lnx-l morphants showed normal expression of boz at the oblong stage (30/30), but in some morphants, boz expression was expanded laterally at 40% epiboly (12/31); 2.5 ng lnx-l UTR MO was injected into each embryo in b and d. e–g, 5-somite stage. e, Uninjected control. f, Embryos injected with 20 ng lnx-l ATG MO; the oval shape indicates dorsalization (92%, N=100). g, boz MO (5 ng), injected together with 20 ng lnx-l ATG MO, alleviated the morphological defects of lnx-l depletion (37% dorsalized, N=116). h–k, gsc expression in embryos injected with indicated MOs. h, uninjected. i, 5 ng of boz MO. j, 2.5 ng lnx-l UTR MO (89% expanded gsc expression, N=28). k, 2.5 ng lnx-l UTR MO plus 5 ng boz MO (32% expanded gsc expression, N=28). l, A schematic model of the role of Lnx-l during establishment of the D–V body axis. Scale bar, 200 µm.