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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Neurobiol Dis. 2009 Mar 28;34(3):487–500. doi: 10.1016/j.nbd.2009.03.004

Figure 3. 14C-DA Conjugation with Mitofilin.

Figure 3

A 14C-DA Cys-CyDye DIGE gel was transferred to PVDF membrane, followed by autoradiography and Western blot analysis to detect mitofilin. The fluorescent scan of the DIGE gel (A), Western blot (B), and autoradiogram (C) of 14C-DA-labeled mitochondrial mitofilin were merged (D) to demonstrate the association between 14C-DA-modification (red), immunodetection (blue), and the DIGE-MS spot identification (green) of mitofilin. Arrows point to the location of mitofilin in (B) and (C).