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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Neurobiol Dis. 2009 Mar 28;34(3):487–500. doi: 10.1016/j.nbd.2009.03.004

Figure 5. Exposure of Differentiated SH-SY5Y Cells to Exogenous 14C-DA Results in DA Conjugation to Cellular Proteins.

Figure 5

Differentiated SH-SY5Y cells were treated with 14C-DA (150μM; 10μCi/mL media) and subjected to 2-D gel electrophoresis, followed by transblotting to PVDF membrane and autoradiography. Samples were analyzed at a pH range of 4-7 pI. Select radioactive spots displaying protein conjugated with 14C-DA were picked from the PVDF membrane and subjected to MS analysis for protein identification. Protein identities are indicated on autoradiogram (details in Table 3). Representative gel of n = 3.