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. 2009 Aug 28;10(10):1140–1146. doi: 10.1038/embor.2009.157

Figure 4.

Figure 4

The NURF complex is required for the transcriptional activation of some Wg targets in Kc cells. Cells were treated with the indicated dsRNAs for 4 days and total RNA were isolated for RT–PCR. The messenger RNA levels of actin, the ribosome protein L49 and TATA binding protein (TBP) were used as internal controls for normalization. Four Wg targets, (A) Nkd, (B) CG5895, (C) CG6234 and (D) Fz3 were measured for basal and activated transcription level. (E) The protein levels of ISWI and α-Tub were monitored in dsRNA-treated cells by Western blot. dsRNA, double-stranded RNA; Fz3, frizzled 3; GFP, green fluorescent protein; ISWI, imitation switch; Nkd, naked cuticle; NURF, nucleosome remodelling factor; RT–PCR, reverse transcription–PCR; Wg, wingless.