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. Author manuscript; available in PMC: 2009 Oct 9.
Published in final edited form as: J Am Chem Soc. 2008 May 21;130(24):7530–7531. doi: 10.1021/ja801479y

Figure 2.

Figure 2

Autoradiogram of a denaturing 20% polyacrylamide gel revealing photocleavage for Rh(bpy)2(chrysi)3+ with the different DNA duplexes. 5′-32P-labeling is on the strand containing 5′-GAC. Conditions are duplex (1 μM), Rh (3 μM) in 50 mM NaCl, 10 mM NaPi, pH 7.1 for 30 min at ambient temperature followed by irradiation (0 or 30 min) with a solar simulator (325–450 nm) and incubation at 60 °C (0 or 30 min). For matched DNA, lanes 1–3 contain DNA with Rh without irradiation, DNA with Rh and 30 min irradiation, and DNA with Rh, 30 min irradiation, and 30 min at 60 °C, respectively. This set of three conditions is repeated for each duplex: MM (lanes 4–6), AB-C (7–9), AB-G (10–12), B-C (13–15), and B-G (16–18). The arrows mark the mispaired site.