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. Author manuscript; available in PMC: 2010 Oct 15.

Published in final edited form as: Free Radic Biol Med. 2009 Aug 7;47(8):1172–1179. doi: 10.1016/j.freeradbiomed.2009.07.036

Fig. 3 — Fig. 3A. Effect of HNE on the recruitment of transcription factors to EpRE of (A) nqo2, (B) gclc and (C) gclm in HepG2 cells. ChIP assays were performed to examined the interaction in vivo of Nrf2, c-Jun and p-c-Jun with EpRE that either treated or untreated with HNE for 3 h. After 3 h stimulation of cells with 10 μM HNE, cells were fixed with1% formaldehyde, lysed, and sonicated to shear chromatin in 0.2–0.8-kb fragments, which were then immunoprecipitated with anti-Nrf2, c-Jun and p-c-Jun antibodies. Pure DNA then analyzed with RT-PCR with specific primers amplifying EpRE elements. Results are reported as mean ± S.E. of at least three determination. *p<0.05, **p<0.01 significantly different compared with control.

Fig. 3B. Effect of HNE on the recruitment of transcription factors to EpRE of (A) nqo2, (B) gclc (EpRE4), (C) gclc (AP-1) and (D) gclm in HBE1 cells. ChIP assays were performed to examined in vivo the interaction of Nrf2, c-Jun and p-c-Jun with EpRE that either treated or untreated with HNE. After 3 h stimulation of cells with 10 μM HNE, cells were fixed with1% formaldehyde, lysed, and sonicated to shear chromatin in 0.2–0.8-kb fragments, which were then immunoprecipitated with anti-Nrf2, c-Jun and p-c-Jun antibodies. Pure DNA then analyzed with RT-PCR with specific primers amplifying EpRE elements. Results are reported as mean ± S.E. of at least three determination. *p<0.05, **p<0.01 significantly different compared with control.

Fig. 3 — Fig. 3A. Effect of HNE on the recruitment of transcription factors to EpRE of (A) nqo2, (B) gclc and (C) gclm in HepG2 cells. ChIP assays were performed to examined the interaction in vivo of Nrf2, c-Jun and p-c-Jun with EpRE that either treated or untreated with HNE for 3 h. After 3 h stimulation of cells with 10 μM HNE, cells were fixed with1% formaldehyde, lysed, and sonicated to shear chromatin in 0.2–0.8-kb fragments, which were then immunoprecipitated with anti-Nrf2, c-Jun and p-c-Jun antibodies. Pure DNA then analyzed with RT-PCR with specific primers amplifying EpRE elements. Results are reported as mean ± S.E. of at least three determination. *p<0.05, **p<0.01 significantly different compared with control.

Fig. 3B. Effect of HNE on the recruitment of transcription factors to EpRE of (A) nqo2, (B) gclc (EpRE4), (C) gclc (AP-1) and (D) gclm in HBE1 cells. ChIP assays were performed to examined in vivo the interaction of Nrf2, c-Jun and p-c-Jun with EpRE that either treated or untreated with HNE. After 3 h stimulation of cells with 10 μM HNE, cells were fixed with1% formaldehyde, lysed, and sonicated to shear chromatin in 0.2–0.8-kb fragments, which were then immunoprecipitated with anti-Nrf2, c-Jun and p-c-Jun antibodies. Pure DNA then analyzed with RT-PCR with specific primers amplifying EpRE elements. Results are reported as mean ± S.E. of at least three determination. *p<0.05, **p<0.01 significantly different compared with control.