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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Free Radic Biol Med. 2009 Aug 7;47(8):1172–1179. doi: 10.1016/j.freeradbiomed.2009.07.036

Fig. 7. Signal pathway involved in HNE induced gclc (EpRE4) and gclm (EpRE) promoters in HepG2 cells.

Fig. 7

HepG2 (A and B) and HBE1 (C and D) cells were transfected with EpRE- gclm (A and C) or EpRE4- gclc luciferase (B and D) promoter plasmids for 24 h and pretreated with JNK inhibitor (JNKi) for 1 h before treated with or without 15 μM HNE. Luciferase activity was determined 24 h after HNE treatment and transfection efficiency was controlled by normalization with cotransfected β galactosidase activity. Results presented are relative to control (pGL-3 promoter luciferase vector). Results are reported as mean ± S.E. of three determination. *p<0.05, **p<0.01 significantly different compared with control. #p<0.05 significantly different compared with treatment.