Fig. 2.
FtsZ-I374V is resistant to both MinCC/MinD and MinC/MinD. One colony of each strain was resuspended in 900 µl of LB medium and serially diluted by 10. Then 3µl culture from each dilution was spotted on plates (with Spc) with or without IPTG (as indicated) and incubated overnight at 37°C. 1, S4/pBANG59 [ftsZ-WT, min∷kan/Ptac∷minCD]; 2, BSM374/pBANG59 [ftsZ-I374V, min∷kan/Ptac∷minCD]; 3, S4/pBANG75 [ftsZ-WT, min∷kan/Plac∷minCCD]; 4, BSM374/pBANG75 [ftsZ-I374V, min∷kan/Plac∷minCCD].