Fig. 5.

The FtsZ-I374V mutant strain is sensitive to MinC^N. (A) BSM374 (ftsZ-I374V, min∷kan) and(B) S4 (min∷kan) harboring the plasmid pZH111 (Para∷malE-minC^N) were grown on plates containing 0.05% arabinose at 37°C. The morphology of these cells was then checked at 5 hours by phase contrast microscopy. As a control, a point mutation (G10D) that inactivates MinC^N was introduced into pZH111 and the mutant protein was expressed in BSM374 (A’) and S4 (B’) with the above conditions.