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. Author manuscript; available in PMC: 2009 Oct 9.
Published in final edited form as: Mol Microbiol. 2009 Apr;72(2):410–424. doi: 10.1111/j.1365-2958.2009.06651.x

Fig. 7.

Fig. 7

Effect of MinCC/MinD induction on the localization of FtsZ, FtsA, ZipA and FtsK. MinCC/MinD was induced with 100 µM IPTG in S4/pBANG75 [ftsZ-WT, min∷kan/Plac∷minCCD]. Cells were removed at indicated time points and analyzed by immunofluorescent microscopy using antisera against FtsA (A1-A3), FtsZ (Z1-Z3), ZipA (P1-P3) and FtsK (K1-K3). As a control, S4/pBANG75 cells were treated with cephalexin at 20 µg/mL for 2 hours to generate filamentous cells. The localization of FtsA (A4), FtsZ(Z4), ZipA(P4) and FtsK(K4) in these filaments was also checked by immuno-staining.