Table 1.
Calculated Reduction Potential for PRL-1 Residuesa
| Residue | E' (mV) | ± SD | δ-15N ox | δ-1H ox | δ-15N red | δ-1H red | Δδ (15N) | Δδ (1H) |
|---|---|---|---|---|---|---|---|---|
| V12 | −366.1 | 0.0 | 126.780 | 9.122 | 126.771 | 9.202 | 0.009 | −0.080 |
| R18 | −364.6 | 2.0 | 121.848 | 5.764 | 121.919 | 5.837 | −0.071 | −0.073 |
| L30 | −364.0 | 1.0 | 124.349 | 7.359 | 124.543 | 7.302 | −0.194 | 0.057 |
| T43 | −363.5 | 0.0 | 111.911 | 7.806 | 112.654 | 7.908 | −0.743 | −0.102 |
| R47 | −361.8 | 0.0 | 126.631 | 8.861 | 129.143 | 8.896 | −2.512 | −0.035 |
| C49 | −364.4 | 0.7 | 121.676 | 8.037 | 112.254 | 6.943 | 9.422 | 1.094 |
| V65 | −365.2 | 0.0 | 124.996 | 8.563 | 125.651 | 8.570 | −0.655 | −0.007 |
| L66 | −364.8 | 0.0 | 130.322 | 9.065 | 130.756 | 8.853 | −0.434 | 0.212 |
| I80 | −364.8 | 1.3 | 119.137 | 7.235 | 119.000 | 6.994 | 0.137 | 0.241 |
| R93 | −364.7 | 2.3 | 118.477 | 7.287 | 118.344 | 7.236 | 0.133 | 0.051 |
| I100 | −364.9 | 2.4 | 127.986 | 8.162 | 127.919 | 8.230 | 0.067 | −0.068 |
| A101 | −364.8 | 1.8 | 129.683 | 9.180 | 129.252 | 8.865 | 0.431 | 0.315 |
| L114 | −365.4 | 2.6 | 116.028 | 7.424 | 117.798 | 7.107 | −1.770 | 0.317 |
| E127 | −363.9 | 1.6 | 118.621 | 9.219 | 119.105 | 9.187 | −0.484 | 0.032 |
| Y152 | −362.7 | 1.9 | 122.770 | 7.129 | 123.362 | 7.099 | −0.592 | 0.030 |
| average | −364.3 | 1.5 | ||||||
a
The reported E’ value for each residue corresponds to the average of both the reduced and oxidized peak heights fit to the equation listed in the methods section. The standard deviation (SD) is also reported in mV. Δδ values were calculated by subtracting the chemical shift of the reduced species from those of the oxidized species.