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. 1998 May 26;95(11):6134–6138. doi: 10.1073/pnas.95.11.6134

Figure 1.

Figure 1

The 3D cryo electron microscope maps of E. coli 70S ribosome complexed with EF-G–GDP–fusidic acid. (A) Side view showing the binding position of EF-G (red) in the intersubunit space. The 30S subunit is on the left and 50S subunit is on the right. EF-G is directly visible and seen to interact with both 30S and 50S subunits (6, 7). The tip of the elongated portion of EF-G is situated in the neck region of 30S subunit, where the anticodon loop region of A site tRNA binds (11, 12). (B) The 30S side view, showing the arc-like structure formed between the EF-G and a protusion at the base of the L7/L12 stalk. Note the clearly defined well-extended L7/L12 stalk. In this view, the tip (domain IV) of EF-G can be seen through the 30S channel (18) where the anticodon of A site tRNA in a pretranslocational complex binds (ref 12; R.K.A., P.P., R.A.G., N. Burkhardt, R. Jünemann, K. H. Nierhaus, and J.F., unpublished results; also see Fig. 4). Landmarks: L1, protein L1 of the 50S subunit; arc, arc-like connection between EF-G and an extension from the stalk base (see text).