Figure 4.

Same as Fig. 3A but showing a magnified view of the site of interaction of different domains of EF-G with the ribosome. Particularly important to note is the partial overlap of domain IV with the A-site tRNA (pink), as obtained from a cryo map of a pretranslocational complex (R.K.A., P.P., R.A.G., N. Burkhardt, R. Jünemann, K. H. Nierhaus, and J.F., unpublished results). Also, note the proximity of the anticodon end of the A site tRNA to the tip of the domain IV. Various domains are labeled with roman numerals and the A-site tRNA is marked as A. Other landmarks are as in Fig. 3.