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RNAi assay in follicular granulosa cells in vitro. Confocal images show the inhibition of EGFP expression in granulosa cells 24 h post transfection. A: Granulosa cells transfected with EGFP plasmid alone; B: EGFP and EGFP-siRNA cotransfection; C: EGFP and Non-silence-siRNA cotransfection; D: Granulosa cells transfected with 6-FAM labeled 125 nM CYP-1.
