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Gonadotrophins induced CYP51 expression in in vitro cultured granulose cells. Both FSH and LH upregulated CYP51 expression time-dependently in follicular granulosa cells, but the peak expression of CYP51 induced by LH (B) (about 12 h after induction) lagged behind FSH (about 8 h after induction) for nearly 4 h (A). The groups with an asterisk (*) show significant difference compared with 0 h, n = 3 (P < 0.05).
