TABLE 1.
siRNA Sequences |
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Name | Target | 5′-3′ | 3′-5′ |
CYP-1 | ORF position 326-344 | CCACAUAUUUACUCUCCAADTDT | DTDTGGUGUAUAAAUGAGAGGUU |
CYP-mismatched (CYP-mis) | ORF position 647-665 | GCCGUAUGUGCCGACGCGDTDT | DTDTCGGCAUACACGGCUGGCGC |
Non-silencing (Plant cell derived siRNAs) | Thermotoga maritimia | UUCUCCGAACGUGUCACGUDTDT | DTDTAAGAGGCUUGCACAGUGCA |
EGFP-siRNAs | EGFP | GCAAGCUGACCCUGAAGUUCDTDT | DTDTCGUUCGACUGGGACUUCAAG |
Small interference RNAs (siRNAs) were acquired by In vitro Transcription T7 kit according to producer's protocol (Jingmei Biotech Co., Ltd, Beijing, China). The sequence for CYP51 was taken from Genebank (gi9910171).