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. 2009 Nov;50(11):2164–2172. doi: 10.1194/jlr.M800533-JLR200

TABLE 1.

Small interference RNA sequences

siRNA Sequences
Name Target 5′-3′ 3′-5′
CYP-1 ORF position 326-344 CCACAUAUUUACUCUCCAADTDT DTDTGGUGUAUAAAUGAGAGGUU
CYP-mismatched (CYP-mis) ORF position 647-665 GCCGUAUGUGCCGACGCGDTDT DTDTCGGCAUACACGGCUGGCGC
Non-silencing (Plant cell derived siRNAs) Thermotoga maritimia UUCUCCGAACGUGUCACGUDTDT DTDTAAGAGGCUUGCACAGUGCA
EGFP-siRNAs EGFP GCAAGCUGACCCUGAAGUUCDTDT DTDTCGUUCGACUGGGACUUCAAG

Small interference RNAs (siRNAs) were acquired by In vitro Transcription T7 kit according to producer's protocol (Jingmei Biotech Co., Ltd, Beijing, China). The sequence for CYP51 was taken from Genebank (gi9910171).