Fig. 4.

Dilation of the rER in PC-depleted MT58 cells is influenced by microbule activity and can be restored by addition of lysoPC. To study the reversibility of the formed ER sphericles, MT58 cells stably expressing ER-YFP were cultured at 33°C (A) or 40°C (B) for 24 h. To cells cultured at 40°C for 24 h, 50 µM lysoPC was added for another 24 h at 40°C (C). To examine microtubule involvement in the formation of ER sphericles, MT58 ER-YFP cells were treated with 10 µM nocodazole for 24 h at 33°C (D), 40°C (E), or cultured at 40°C without nocodazole treatment (F). Bar = 10 µm.