TABLE 2.
Expression of SR-BI and CD36 in mouse small intestine
| SR-BI/18sbc |
CD36/18sbc |
||||
|---|---|---|---|---|---|
| Mouse Genotype | Intestinal Dectiona | Basal Diet | HFHC Diet | Basal Diet | HFHC Diet |
| WT | A | 1.0 | 1.49 ± 0.09 | 1.0 | 1.05 ± 0.05 |
| B | 0.07 ± 0.01 | 0.05 ± 0.01 | 0.69 ± 0.08 | 0.88 ± 0.07 | |
| C | 0.14 ± 0.02 | 0.13 ± 0.02 | 0.39 ± 0.03 | 0.63 ± 0.06 | |
| SR-BI−/− | A | 0.66 ± 0.07 | 1.02 ± 0.06 | ||
| B | 0.56 ± 0.06 | 0.84 ± 0.05 | |||
| C | 0.29 ± 0.07 | 0.59 ± 0.05 | |||
| CD36−/− | A | 0.28 ± 0.03 | 1.07 ± 0.02 | ||
| B | 0.17 ± 0.02 | 0.29 ± 0.04 | |||
| C | 0.10 ± 0.01 | 0.13 ± 0.01 | |||
mRNA was extracted from pooled intestinal segments (A = proximal 10 cm, B = medial 10 cm, and C = distal 10 cm; see Methods) of mice (n = 3 per group) fed either a basal diet or HFHC diet for 3 weeks and analyzed by real-time PCR.
The relative mRNA levels from at least two real time PCR experiments are normalized to the value for intestinal segment A from WT mice fed a basal diet.
Mean ± SD, n = 6–12. ANOVA followed by the Dunnett multiple comparison test indicates that all SR-BI relative mRNA levels are significantly different (P < 0.05) to the control value for intestinal segment A from WT mice fed a basal diet. The same applies to the CD36 relative mRNA levels except that the values for intestinal segment A from WT and SR-BI−/− mice fed a HFHC diet are not significantly different to control.