TABLE 3.
Effect of SR-BI and CD36 deletion on expression of NPC1L1 and ABCG5 in mouse small intestine
| Mouse Genotype | Intestinal Section | NPC1L1/18sab | ABCG5/18sab |
|---|---|---|---|
| WT | A | 1.0 | 1.0 |
| B | 0.94 ± 0.06 | 0.59 ± 0.04 | |
| C | 2.02 ± 0.1 | 0.75 ± 0.07 | |
| SR-BI−/− | A | 0.50 ± 0.03 | 0.32 ± 0.01 |
| B | 0.92 ± 0.06 | 0.46 ± 0.04 | |
| C | 0.84 ± 0.05 | 0.33 ± 0.02 | |
| CD36−/− | A | 0.60 ± 0.04 | 0.51 ± 0.03 |
| B | 1.12 ± 0.01 | 0.68 ± 0.08 | |
| C | 0.86 ± 0.06 | 0.35 ± 0.03 | |
| SR-BI−/−/CD36−/− | A | 0.46 ± 0.06 | 0.37 ± 0.03 |
| B | 0.94 ± 0.05 | 0.58 ± 0.06 | |
| C | 1.44 ± 0.19 | 0.51 ± 0.03 |
mRNA was extracted from the pooled small intestines of mice (n = 3 per group) fed a basal diet for 3 weeks and analyzed by real-time PCR. The relative mRNA levels (mean ± SD, n = 9–12) for the small intestine segments (cf. Table 2) are from at least two real-time PCR experiments and are normalized to the values for intestinal segment A from WT mice.
ANOVA followed by the Dunnett multiple comparison test indicates that all NPC1L1 relative mRNA levels, except those for intestinal segment B from WT, SR-BI−/−, and SR-BI−/− / CD36−/− mice, are significantly different (P < 0.05) to the control value for intestinal segment A from WT mice fed a basal diet. All ABCG5 relative mRNA levels are significantly different to the control value for intestinal segment A of WT mice fed a basal diet.