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. 2009 Nov;50(11):2258–2264. doi: 10.1194/jlr.M900107-JLR200

Fig. 4.

Fig. 4.

25-Hydroxycholesterol treatment of macrophages induced CCL5 expression. Mouse macrophage-like cells were treated under various conditions and the CCL5 released into the culture media was measured by ELISA. A: The mouse macrophage-like cell line RAW264.7 (in DMEM with 10% FCS) was treated with 25-hydroxycholesterol for 18 h. B: RAW264.7 cells (in DMEM with 10% FCS) were pretreated with 25-hydroxycholesterol for 30 min before treatment with 10 ng/ml LPS for 18 h. C: RAW264.7 cells (in DMEM with 2% FCS) were treated with varying concentrations of 25-hydroxycholesterol for 18 h. Average data ± SD are presented. ** P < 0.01 in a one-way ANOVA with Dunnett’s multiple testing correction compared with untreated cells (n = 4 individual cultures). Culture medium contained <1 ng/ml 25-hydroxycholesterol.