Fig. 2.

HSL and Plin form a physical interaction. Full-length Plin was in vitro translated with [³⁵S]methionine using the TNT transcription/translation system (lane 1). Extracts (400 μg) of baculovirus overexpressing GST (lane 2) or GST-HSL fusion protein (GST-HSL, lane 3) were incubated with glutathione-agarose beads and with [³⁵S]methionine-labeled Plin as described in Experimental Procedures. Proteins that bound to the beads were eluted, separated on SDS/4–20% PAGE, and visualized using a PhosphorImager.