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In vitro proteasome-inhibitory activity of 1-3 in C4-2B cell extracts: (a) Proteasomal chymotrypsin-like activity (%CT) of the ligand HLI, the salt CuCl2, and an 1:1 HLI:CuCl2 mixture in DMSO at 25 mmol/L stock solution. (b) Concentration dependent inhibition by 1-3 at 0.1, 1, 5, 10, and 25 μM.
