Table 1.
Primers used to create the RARαγ and RXRα-R chimeric constructs with the engineered restriction sites or mutations underlined.
Construct | Primer sequence | |
---|---|---|
αγ1 | Forward | 5′-TGCCAGCTGGGCAAGTATACTACGAA-3′ BstZ17I |
Reverse | 5′-CCGCAGGATCAGGATATCCAGGCA-3′ EcoRV | |
αγ2 | Forward | 5′-GCCTGCCTGGATATCCTGATCCTG-3′ EcoRV |
Reverse | 5′-TTGGGGAACATGTGGGGGCGGCT-3′ PciI | |
RXRα-R | Forward | 5′-CTCTTCTTCTTCAAGCTCATCGGGGACACACCCATTGACCCTCTCATCCAGGAGATGCTGGAGGCGCCGCACCAAATGACTTAG-3′ |
Reverse | 5′-CTAAGTCATTTGGTGCGGCGCCTCCAGCATCTCCTGGATGAGAGGGTCAATGGGTGTGTCCCCGATGAGCTTG AAGAAGAAGAG-3′ |