TABLE 1.
Microarray Quality Controls
| A. GAPDH Average Sis (n = 4) SI SP/nSP Ratio as a Function of Relative Distance of Probed Transcript from the Start of the RNA Message Poly A Sequence (position 1261) | |||
|---|---|---|---|
| GAPDH Probe Location 5′–3′/(dist. polyA) |
SP-SI | SP/nSP | nSP-SI |
| 908–1256/ (5–353) | 9132 | 0.92 | 9898 |
| 493–832/(429–769) | 4793 | 0.81 | 5918 |
| 87–387/(874–1164) | 2159 | 0.93 | 2317 |
| B. Number of P Calls in the SP and nSP Cells in Each Microarray-Paired Experiment | |||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | Mean ± SD | |
| SP-P | 23854 | 22174 | 22561 | 22310 | 22725 ± 770 |
| nSP-P | 20510 | 20570 | 20032 | 19691 | 20746 ± 449 |
| Δ(SP-nSP) | 3344 | 1604 | 2529 | 2629 | 2419 ± 718 |
| C. Signal Intensities and P and A Calls across the Microarray- Paired Experiments for the Lineage Markers | ||||
|---|---|---|---|---|
| SP | nSP | |||
| Marker | Aver. SI | P/A-1234 | Aver. SI | P/A-1234 |
| Tyrosinase | 28 | AMAA | 17 | AAPA |
| CD45 | 18 | PAAP | 12 | AAAA |
| CD1a | 16 | AAAA | 31 | AAAA |
| ΣKeratins | 3384 | 3485 | ||