Table 1.
miRNA targeting of neural progenitor maintenance genes
| Target Gene | ||||||
| miRNA ID | Fgf2 | Fgfr1 | Efnb1 | Rgs3 | Notch1 | Mef2d |
| mmu-let-7 family | (2..8) CUACCUC | |||||
| mmu-miR-15b and 16 | (1..7) GCUGCUA × 2 | (2..8) UGCUGCU | (1..7) GCUGCUA | |||
| mmu-miR-17 and 20a | (2..8) GCACUUU | |||||
| mmu-miR-25 | (2..8) GUGCAAU | (2..7) UGCAAU | (2..8) GUGCAAU | |||
| mmu-miR-30c | (1..7) GUUUACA | |||||
| mmu-miR-103 | (1..8) AUGCUGCU | (2..7) UGCUGC | (2..8) AUGCUGC | |||
| mmu-miR-124 | (2..8) GUGCCUU | |||||
| mmu-miR-125 family | (1..7) UCAGGGA | |||||
Evolutionarily conserved sites complementary to seed regions of highly expressed miRNAs (among top 30 most expressed) were found in 3'UTRs of the genes previously shown to be involved in maintenance of neural progenitors: Fgf2 - fibroblast growth factor 2 [35], Fgfr1 - fibroblast growth factor receptor 1 [35], Efnb1 - ephrin B1 [36], Rgs3 - PDZ-RGS3 [36], Notch1 [34] and Mef2d - myocyte enhancer factor 2D [70]. RNA sequences in uppercase represent the seed matching sites (from TargetScan [21]) of aligned 3'UTRs. All sequences are conserved between human, mouse, rat and dog. Numbers in parentheses show the the positions of mature miRNA sequences to which the 3'UTR sites are complementary.