Figure 4.
The effect of dBcAMP on dystrophin, Dp71, and utrophin mRNA expression in cultured astrocytes. Five micrograms of poly(A)-rich RNA from rat cultured astrocytes was separated by electrophoresis, transferred to a nylon membrane, and hybridized with 32P-labeled rat Dp71 (panels a and a′) PCR products and utrophin (panel b) cDNA. The hybridization signals (arrows) were visualized following autoradiography for 18 hr (panels a and b) and 2 hr (panel a′) by use of a BAS2000 image analyzer. RNA samples: astrocytes cultured for 6 days in the absence (−) and presence (+) of 1 mM dBcAMP. The filters used for panels a and a′ were the same. The membrane was rehybridized with a 32P-labeled human glyceraldehyde-3-phosphate dehydrogenase (G3PDH) cDNA probe to correct for the amount of RNA loaded in each lane.