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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2009 Sep 1;8(9):2746–2753. doi: 10.1158/1535-7163.MCT-09-0273

Figure 2.

Figure 2

CXCR4 is required for chemotactic migration and invasion of BM-NSCs towards glioma secreted factors. A, Immunostaining showing expression of CXCR4 by BM-NSCs. B, FACS analysis indicating CXCR4 expression in cultured BM-NSCs. (left) isotype control. (right) anti-CXCR4 antibody. C, BM-NSCs migration towards 1:10 diluted glioma-conditioned medium is inhibited in the presence of a blocking anti-CXCR4 antibody. Stem cell growth medium and the medium containing 10% FBS were used as controls, respectively. D, (top) Representative pictures showing BM-NSCs invasion towards medium (a), medium with 10% FBS (b), medium with 1:10 glioma-conditioned medium in the absence (c) and presence (d) of a blocking anti-CXCR4 antibody, respectively. (bottom) Quantification of cell invasion indicated that cell invasion towards glioma-conditioned medium is inhibited when CXCR4 is blocked. * p<0.05.