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. Author manuscript; available in PMC: 2009 Oct 11.
Published in final edited form as: Chem Res Toxicol. 2008 Jan 31;21(2):432–444. doi: 10.1021/tx700347w

Figure 7.

Figure 7

Streptavidin purification of Al-HNE adducted proteins conjugated with biotin. Cellular lysates (Lane 1) containing Al-HNE adducted proteins conjugated with biotin (2 mg) were incubated with 1 ml of SA beads (1:1 in ammonium bicarbonate) for 2 h. After 2 h the beads were recovered by centrifugation and the breakthrough was collected (Lane 2). Successive washes include 1% SDS (Lane 3), 4 M urea (Lane 4), and 1 M NaCl (Lane 5). Elution of biotin was done with 70% CH3CN, 5% formic acid (Lane 6). Biotin tagged proteins were detected with HRP-SA and 2.5 μg of protein was loaded per lane. Panel A shows control (DMSO) and 5 μM Al-HNE and panel B shows 10 and 50 μM Al-HNE. S=biotin labeled standard curve.