Table 1.
[35S]TBPS binding and its modulation by GABA and THIP in frontal sections of the rat brain as revealed by quantitative autoradiography.
| Brain area | Basal binding (nCi/mg) |
GABA 10mM (% of basal) |
THIP 1mM (% of basal) |
|---|---|---|---|
| Olfactory bulb | 295±36 | 3.1±0.6 | 3.4±0.7 |
| Cerebral cortex, external layer | 543±66 | 1.3±0.2 | 1.1±0.2b |
| Cerebral cortex, internal layer | 1026±73 | 0.9±0.1 | 1.2±0.2a |
| Anterior cortical amygdaloid nucleus | 1840±261 | 1.1±0.2 | 2.0±0.2c |
| Medial amygdaloid nucleus | 1535±198 | 1.0±0.2 | 2.3±0.4b |
| Anterior amygdaloid area | 2049±219 | 1.1±0.2 | 2.0±0.3b |
| Caudate-putamen anterior | 630±42 | 0.9±0.1 | 1.5±0.2b |
| Caudate-putamen posterior | 523±23 | 1.1±0.1 | 1.7±0.1b |
| Globus pallidus | 1704±158 | 0.7±0.1 | 2.6±0.2c |
| Globus pallidus-ventral pallidum | 1641±164 | 0.8±0.1 | 2.5±0.2c |
| Globus pallidus-basal nucleus of Meynert | 1395±94 | 0.8±0.1 | 2.8±0.2c |
| Nucleus vertical limb of diagonal band | 1787±147 | 2.1±0.2 | 2.1±0.2 |
| Nucleus horizontal limb of diagonal band | 1819±150 | 1.6±0.1 | 2.5±0.3a |
| Lateral preoptic area | 1527±167 | 1.2±0.1 | 1.6±0.3 |
| Lateral hypothalamus | 1375±166 | 0.7±0.1 | 1.6±0.3b |
| CA3 area of hippocampus | 510±59 | 1.5±0.2 | 1.4±0.2 |
| CA4 area of hippocampus | 541±40 | 1.3±0.2 | 2.3±0.3c |
| Reticular nucleus of thalamus | 82±9 | 4.3±2.6 | 20±6b |
| Gelatinosus nucleus of thalamus | 1334±125 | 3.0±0.4 | 2.6±0.2 |
| Ventrolateral thalamic nucleus | 1049±105 | 3.4±0.6 | 2.6±0.3 |
| Ventroposterolateral thalamic nucleus | 907±88 | 4.2±0.5 | 3.0±0.5b |
| Medial geniculate nucleus | 1030±29 | 2.6±0.2 | 2.8±0.2 |
| Substantia nigra, reticular part | 1487±115 | 0.8±0.2 | 1.8±0.1b |
| Paranigral nucleus | 1028±123 | 1.0±0.2 | 3.0±0.7b |
| Oculomotor nucleus | 1198±138 | 1.3±0.2 | 3.4±0.4c |
| Nucleus lateral lemniscus dorsal/ventral | 1452±190 | 1.4±0.2 | 2.6±0.4b |
| Superficial grey layer of superior colliculus | 1642±121 | 1.4±0.2 | 3.0±0.3b |
| Inferior colliculus | 1701±119 | 1.2±0.1 | 2.4±0.2c |
| Olivary pretectal nucleus | 1279±131 | 2.4±0.2 | 2.9±0.3a |
| Anterior pretectal area | 1808±80 | 1.5±0.2 | 3.1±0.3b |
| Central gray | 1076±93 | 1.0±0.1 | 2.1±0.3a |
| Brain stem | 493±71 | 1.6±0.1 | 9.2±1.9b |
| Granule cell layer of cerebellum | 281±26 | 7.1±0.6 | 4.6±0.4b |
The values (mean ± SEM, n = 9) of basal binding are shown as nCi/mg, others are per cent values of the corresponding basal [35S]TBPS binding.
p < 0.05 for the significance of difference between the proportions of remaining [35S]TBPS binding after application of THIP compared to GABA (paired t-test).
p < 0.01 for the significance of difference between the proportions of remaining [35S]TBPS binding after application of THIP compared to GABA (paired t-test).
p < 0.001 for the significance of difference between the proportions of remaining [35S]TBPS binding after application of THIP compared to GABA (paired t-test).