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. 2009 Oct 21;4(10):e7545. doi: 10.1371/journal.pone.0007545

Figure 2. Comparison of live Nile Red staining, fixed Nile Red staining, and triacylglycerol stores in wild type and mutants.

Figure 2

Triacylglycerol (TAG) stores were determined by TLC/GC of lipid extracts. %TAG refers to the percentage of total fatty acid detected in the TAG fraction. Anterior is to the left. Nile Red fed to live worms accumulates in gut granules, which are lysosome-related organelles. After worms are fixed in paraformaldehyde, the intensity and size of Nile Red staining-droplets correlates well with the biochemical determinations of TAG stores.