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. 2009 Oct 21;4(10):e7534. doi: 10.1371/journal.pone.0007534

Figure 2. In silico prediction and functional characterization of promoter elements in intron 5 of human DCLK1.

Figure 2

(A) Illustration of probability scores for transcription factor binding-sites in three clusters and a promoter proximal region (merged pictures, see SOM). Arrows indicate the position of SNP markers. See SOM for cis1-3 and promoter chromosomal coordinates. (B) Schematic representation of luciferase reporter vectors. Abbreviations: Prom, promoter; luc, luciferase reporter gene. (C) Luciferase reporter assay demonstrating basal promoter activity and allele-specific responses to neuronal differentiation of SH-SY5Y cells. (D) Luciferase reporter assays demonstrating cis2 haplotype-specific effects in response to neuronal differentiation of SH-SY5Y cells. Luciferase signal intensities obtained from non-differentiated cells (grey bars) and RA/BDNF-exposed cells (black bars) are compared for each reporter plasmid. Values are given as mean ± S.E.M. * Statistically significant different expression in differentiated cells as compared to control cells (t-test, p<0.05).