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. 2009 Aug 13;28(19):2919–2931. doi: 10.1038/emboj.2009.229

Figure 2.

Figure 2

Transient activation of Snf1 following UV irradiation. (A and B) Phosphorylation of Snf1 Thr 210 after UV irradiation. HA-tagged Snf1 was immunoprecipitated from whole cell extracts and western blots of the precipitated material were probed for Snf1–HA or the phosphorylated form of Snf1 (PT210). Samples were taken 2 min after irradiation (A) or at the indicated times (B). Cells switched to either fresh glucose- or galactose-containing medium for 1 h were controls for Snf1 activation (B, lanes 7 and 8). U, untagged control. (C) Activation of human AMPK in response to UV irradiation. HEK-293T cells were irradiated and collected at the indicated time points. Western blotting was carried out for total AMPK and the phospho-T172 form of the enzyme. Growth for 4 h in the absence of glucose was used as a positive control for AMPK activation (lane 6).