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. 2009 Aug 13;28(19):2919–2931. doi: 10.1038/emboj.2009.229

Figure 3.

Figure 3

Snf1 kinase activity is important for UV resistance of rad23Δ cells and induction of Snf1-dependent UV responsive genes. (A) UV sensitivity of indicated yeast strains. Percent survival signifies the number of colonies on agar plates after irradiation relative to unirradiated controls. Experiments were done in triplicate and error bars represent the standard deviation (B and C) UV sensitivity of cells harbouring a kinase-dead snf1 point mutant, snf1-K84R, (B), or an activation-deficient snf1 mutant, snf1-T210A, (C). Results were obtained as in (A). (D–F) Expression of three UV-induced, Snf1-dependent genes in snf1Δ cells harbouring plasmid-borne WT SNF1, the kinase-dead snf1-K84R allele, the activation-deficient snf1-T210A allele or vector only control. Expression levels were determined by quantitative RT–PCR using primer sets specific to the FIS1 ORF (D), BOP2 ORF (E), or GAD1 ORF (F). RNA levels were normalized to ACT1 expression and were averaged from three independent experiments. Error bars represent the standard deviation.