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. 2009 Aug 20;28(19):2874–2884. doi: 10.1038/emboj.2009.231

Figure 4.

Figure 4

CTL*myc interacts with Sec61p until the CPY* moiety of CTL*myc is degraded. Cells of the yeast strains Δprc1 and Δprc1Δhul5 expressing either plasmid encoded CTL* or CTL*myc were treated with CHX for 0 and 3.5 h. This enables the accumulation of the fragment truncCTL*myc in a Δprc1Δhul5 strain (lane 7). Solubilized microsomal proteins were subjected to immunoprecipitation using α-myc antibodies (α-myc precipitates) and were analysed by immunoblot analysis with the indicated antibodies. Except for the interaction studies of Sec61p, this figure was earlier published in Kohlmann et al (2008). CTL*myc interacts with Sec61p during its degradation (lanes 11 and 12) as well as the known components of the ERAD machinery, Der3p and Cdc48 (Kohlmann et al, 2008).