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. 2009 Aug 20;28(19):3005–3014. doi: 10.1038/emboj.2009.235

Figure 6.

Figure 6

RECQ4–MCM complex is required for replication-restart after hydroxyurea treatment. (A) Flow cytometry of 293T cells 48 h after transfecting with mock, CDC6, MCM7, MCM10 and RECQ4 siRNA. (B) Outline of 3H-thymidine labelling of the siRNA knockdown cells after hydroxyurea treatment. (C) DNA synthesis of 293T siRNA knockdown cells after hydroxyurea treatment was monitored by [3H]-thymidine labelling. Count per minute (CPM) was divided by the actual number of cells of each of the siRNA knockdowns to obtain CPM per cell (y-axis).