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. 2009 Oct 21;4(10):e7535. doi: 10.1371/journal.pone.0007535

Figure 5. Targeting of nephronectin with miR-378.

Figure 5

(A) Computational algorithms were used to predict the miRNA that binds to a target sequence in the 3′-UTR of nephronectin. miR-378 was found to have the strongest binding to the 3′UTR of nephronectin from nucleotides 1902–1923. (B) Expression of endogenous miR-378 in MC3T3-E1 cells under differentiation-inducing conditions was measured by real-time PCR. (C) Left, MC3T3-E1 cells stably transfected with the miR-378 construct were grown in parallel with cells transfected with the control vector for 10 days and stained for ALP activity. Multiple fields were examined for each construct, and stained cells were counted per field for quantitative analysis. Right, typical micrographs of ALP expression are shown in two separate stable clones.