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. 2009 Oct 21;4(10):e7535. doi: 10.1371/journal.pone.0007535

Figure 9. Npnt 3′UTR competes with GalNT7 3′UTR for miR-378 binding.

Figure 9

(A) U343 cells were transiently transfected with luciferase reporter vector harboring the GalNT7 3′UTR (lu-Gal-UTR) and different constructs at the amounts indicated on the figure (ng). Luciferase activities were normalized using the control as 100%. The luciferase activities were higher when co-transfected with Npnt+3′ as compared with Npnt+3′-mu, suggesting that increased supplies of Npnt+3′ absorbed some endogenous miRNAs freeing luciferase translation. (B) Diagram showing competition of Npnt+3′, but not Npnt+3′-mu, with GalNT7 for miR-378 binding. As a result, luc-Gal-UTR was freed from miR-378 binding and thereby having higher luciferase activities. (C) Western blot showing increased expression of GalNT7 in the Npnt+3′-transfected cells compared with the Npnt-transfected cells.