Table 1. Summary of the results from the methods used to deadsorb PrP from mte and kte.
Desorption Solution | Temperature (°C) | Amount of mPrP released from mte | Amount of mPrP released from kte | ||
mg | % | mg | % | ||
Sodium acetate pH 3 | 25 | 0.05 | 5 | 0.01 | 1 |
Sodium acetate pH 5 | 25 | 0.04 | 4 | 0.01 | 1 |
MES/Tris pH 8 | 25 | 0.09 | 9 | 0.03 | 3 |
MES/Tris pH 10 | 25 | 0.09 | 9 | 0.05 | 5 |
10% SDS | 25 | 0.18 | 18 | 0.09 | 9 |
Desorption buffer | 25 | 0.39 | 39 | 0.20 | 20 |
2 M Sodium Chloride | 25 | 0.13 | 13 | 0.07 | 7 |
MilliQ water | 25 | 0.01 | 1 | n/d | n/d |
Sodium acetate pH 3 | 100 | 0.14 | 14 | 0.11 | 11 |
Sodium acetate pH 5 | 100 | 0.10 | 10 | 0.10 | 10 |
MES/Tris pH 8 | 100 | 0.11 | 11 | 0.09 | 9 |
MES/Tris pH 10 | 100 | 0.14 | 14 | 0.10 | 10 |
10% SDS | 100 | 0.28 | 28 | 0.24 | 24 |
Desorption buffer | 100 | 0.95 | 95 | 0.70 | 70 |
2 M sodium chloride | 100 | 0.24 | 24 | 0.21 | 21 |
MilliQ water | 100 | 0.05 | 5 | 0.08 | 8 |
n/d–not detected.