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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 16;386(3):488–492. doi: 10.1016/j.bbrc.2009.06.064

Figure 3. Expression of CAIX and tubulin in PC-3 prostate cancer cells.

Figure 3

PC-3 cells were cultured under normoxic or hypoxic conditions (1% O2 for 48 hours). Cell lysates (15ug) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Blots were probed with antibodies to tubulin (1:1000) and CAIX (NB100 and M75, both at 1:5000 dilution).