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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 16;386(3):488–492. doi: 10.1016/j.bbrc.2009.06.064

Figure 4. Detection of CAIX in xenograft tumors grown from PC-3 cells in mice.

Figure 4

Mice were injected subcutaneously in the flank with 3×106 PC-3 prostate cancer cells and tumors were resected on Day 39. Immunohistochemical staining of Formalin-fixed, paraffin-embedded sections was performed using antibodies to CAIX (NB100 at 1:1000, 1:3000 and 1:5000 dilutions; M75 at 1:5000 dilution). Antigen retrieval was performed by incubating slides in 10 mM citric acid buffer pH 6.0, as described in Methods. Stained sections were photographed at using a Zeiss Axioplan 2 imaging microscope and Openlab 5.0.3 Beta Improvision® software.