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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Mol Cell. 2009 Sep 24;35(6):889–902. doi: 10.1016/j.molcel.2009.09.011

Figure 1. Identification of Factor-nucleosome Interactions in vivo.

Figure 1

(A) Diagram outlining the factor-nucleosome interaction assay. Cells encoding a TAP-tagged protein are treated with formaldehyde. Chromatin is then isolated and solubilized to mononucleosomes with MNase, then subjected to TAP purification. Adapter capture of mono-nucleosomal DNA produces an ~200 bp LM-PCR product, which can be subsequently mapped genome-wide.

(B) LM-PCR detection of the indicated factor-nucleosome interaction. BY4741 is a negative control that lacks the TAP tag; H2A.Z is a positive control. Input represents the equivalent of 10−5 ml of cell culture used in LM-PCR, and is the material used for the immunoprecipitation. The volume of cell culture (at OD600 = 0.8) used in the TAP purification is indicated.

(C) Bdf1-nucleosome interactions are dependent on Nua4 (Esa1)-directed acetylation. LM-PCR experiments were performed on the indicated strains as described in panels A and B.