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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Apr 25;371(4):644–648. doi: 10.1016/j.bbrc.2008.04.080

FIG. 1.

FIG. 1

FIG. 1

FIG. 1

A SH3BP2 INCREASES THE NUCLEAR TRANSLOCATION OF NFATC1. RAW 264.7 cells were transiently transfected with murine SH3BP2 (red) and NFATc1 (green) translocated to the nucleus (A) compared to cells not transfected with SH3BP2 (B). SH3BP2 did not increase the total amount of NFATc1 in cells however SH3BP2 with sRANKL (100ng/ml) increased NFATc1 activation by increasing the translocation.

C. SH3BP2 INCREASES NFAT ACTIVITY. The figure demonstrates that in RAW 264.7 cells stimulated with sRANKL (100ng/ml), murine SH3BP2 cDNA significantly increases NFAT activity compared to the pMT3 vector only (p<0.0003), sRANKL (p<0.0001) or SH3BP2 alone (p<0.0001). This experiment was repeated four times with similar results.

D. TRAP ACTIVITY IS INCREASED IN THE PRESENCE OF SH3BP2. RAW 264.7 cells were transiently transfected with murine SH3BP2 and stimulated with sRANKL (100ng/ml). In RANKL stimulated (100ng/ml) and SH3BP2 transfected cells TRAP activity was significantly increased compared to the vector only (pMT3 vector) control p<0.0001, SH3BP2 alone (p<0.0001) and sRANKL stimulation alone (p=0.001). # indicates p<0.02 and ## indicates p<0.0001. This experiment was repeated three times with similar results.