Figure 1. FLIPR analysis of changes in intracellular calcium in cultured rat urothelial cells by purinergic receptor agonists and antagonists.
(A) Illustrates the concentration-response (100 nM to 1 mM) of a range of purinergic receptor agonists in cultured rat urothelial cells. (B, C) Effect of P2 receptor antagonists, PPADS (30 μM), suramin (30 μM) and MRS2179 (30 μM) on ATP and UTPγS evoked changes in intracellular calcium in cultured rat urothelial cells. (D) Representative continuous traces of changes in fluo-3 fluorescence in response to UTPγS (100 μM) alone and in the presence of PPADS (30 μM).