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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: J Neurosci Res. 2008 Jul;86(9):2100–2110. doi: 10.1002/jnr.21653

Figure 5. Effects of Tat and morphine on MOR mRNA expression in microglia.

Figure 5

Cultured (A) N9 microglial cells and (B) primary microglia were grown as described in Methods, and treated with vehicle, 50 nM Tat and/or 500 nM morphine for 24 h as described in Methods. Cells were then harvested, mRNA extracted, and MOR expression was analyzed by real-time PCR as described in Methods. Data were analyzed by ANOVA, and *, **, *** indicate statistically significant differences (p<0.05, p<0.01, and p<0.001, respectively) in mRNA expression compared to vehicle-treated (control) microglia.