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. Author manuscript; available in PMC: 2009 Oct 12.
Published in final edited form as: J Immunol Methods. 2007 Apr 9;323(2):101–108. doi: 10.1016/j.jim.2007.03.005

Figure 2. Expression of costimulatory molecules and pro-inflammatory cytokine of serum-free cultured DCs.

Figure 2

DCs were grown in different culture media as described. DCs were further stimulated as indicated on day 6 for 48 hours (a) or 16-18 hours (b). (a) DCs were exposed to inflammatory stimuli, washed with PBS, and stained with fluorescence conjugated antibodies to CD11c, CD80, CD86, and I-Ad. MFI of CD80, CD86, and I-Ad was calculated from CD11c-positive cells exclusively. (b) DCs were plated with inflammatory stimuli indicated in 24 well plates at 106/ml for 16-18 hours. Supernatants were harvested and cytokines were measured by Sandwich ELISA. Results represent mean concentration ± SD of 5 individual experiments.