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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(6):2375–2383. doi: 10.1158/0008-5472.CAN-08-3359

Figure 2.

Figure 2

MCF7 subclones exhibiting mitochondrial dysfunction had more aggressive cellular behaviors than did MCF7 cells. A, MCF7 subclones migrated faster than MCF7 cells, as shown in a wound healing assay. The confluent monolayer of cells was pretreated with 5 mmol/L NAC for 3 h (as indicated) and then wounded by scraping a narrow 200-µL pipette tip across the plate. Every day, each well was examined by phase-contrast microscopy for the amount of wound closure, and wound images were captured under a phase-contrast Nikon microscope (Nikon Eclipse TE300). B, MCF7 subclones migrated faster than MCF7 cells through uncoated membranes in modified Boyden chamber assays. C, MCF7 subclones were more invasive than MCF7 cells. Cells were cultured on transwell cell culture inserts coated with Matrigel. Results of a representative experiment (n = 3). D, histologic morphology of H&E-stained tissue slices of clone P tumor and metastasis.