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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(6):2375–2383. doi: 10.1158/0008-5472.CAN-08-3359

Figure 3.

Figure 3

CXCL14 promotes cell motility. A, analysis of CXCL14 expression in MCF7 cells and subclones by RT-PCR (top) and by Western blotting (bottom). B, addition of exogenous CXCL14 promoted MCF7 cell migration in vitro . Plated MCF7 cells were grown to confluency, wounded, and then incubated with either 0.57 ng CXCL14 or conditioned medium from H or P clones for 48 h. Each plate was examined by phase-contrast microscopy for the amount of wound closure. C, transient transfection of CXCL14 promotes MCF7 cell migration in vitro . MCF7 cells were transfected with 0.5 µg of CXCL14 plasmid. After 48 h, cells were collected and used in a migration assay. D, inhibition of invasion of clone H by AP-1 decoy. Clone H cells were transfected with either AP-1decoy or scramble decoy (100 pmol). After 48 h, cells were collected and lysed and CXCL14 expression was analyzed by Western blotting (left ), or cells were collected and cultured on transwell cell culture inserts coated with Matrigel for an additional 20 h (right). Results of a representative experiment (n = 3).