Table I.

Mitochondrial Targeting Scores for Long Mouse SIRT3 with Three Different Starting Methionine Residues (Met1, Met15, and Met78), as well as for SIRT3 of Human, Rat, and Rabbit

	Mouse SIRT3			Rat SIRT3		Human SIRT3	Rabbit SIRT3
	1–334 aa	15–334 aa	78–334 aa	1–334a aa	1–320 aa	1–399 aa	1–319 aa
MitoProt II	0.6043	0.7847	0.0086	0.5418	0.7376	0.7455	0.941
Predotar	0	0.54	0	0.01	0.4	0.65	0.78
TargetP	0.12	0.814	0.18	0.109	0.819	0.555	0.938

Three different mitochondria targeting prediction programs were used.

Scores range from 0 to 1, with a higher score indicating higher probability for the protein to contain a mitochondria targeting sequence.

^aIndicates the rat SIRT3 that was predicted from the rat genome, but was not cloned.