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. 2009 Jan 6;18(3):549–558. doi: 10.1002/pro.54

Table II.

DNA binding Properties of TthUVDE wt and Mutants Tested in a Filter Binding Assay

% Binding
% Binding
DNA lesion UVDE no Mn2+ 1 mM Mn2+
CPD Wild type 34 ± 2 33 ± 1.7
CPD K229L 31 ± 0.7 31 ± 0.7
CPD K229R 31 ± 0.5 32 ± 0.2
6-4PP Wild type 19 ± 3.7 20 ± 1
6-4PP K229L 20 ± 0.5 21 ± 1.8
6-4PP K229R 17 ± 2.8 19 ± 0.7
Abasic site Wild type 20 ± 2 23 ± 0.7
Abasic site K229L 21 ± 0.7 21 ± 1.4
Abasic site K229R 21 ± 2 21 ± 0.7
No damage Wild type 2.5 ± 0.1 2.8 ± 0.4
No damage K229L 3.7 ± 0.2 3.8 ± 0.4
No damage K229R 7.6 ± 0.7 5.8 ± 1

Binding is expressed as the percentage of the input DNA retained on the filter. Binding efficiencies were tested in presence and absence of 1 mM MnCl2.