Table II.
% Binding |
% Binding |
||
---|---|---|---|
DNA lesion | UVDE | no Mn2+ | 1 mM Mn2+ |
CPD | Wild type | 34 ± 2 | 33 ± 1.7 |
CPD | K229L | 31 ± 0.7 | 31 ± 0.7 |
CPD | K229R | 31 ± 0.5 | 32 ± 0.2 |
6-4PP | Wild type | 19 ± 3.7 | 20 ± 1 |
6-4PP | K229L | 20 ± 0.5 | 21 ± 1.8 |
6-4PP | K229R | 17 ± 2.8 | 19 ± 0.7 |
Abasic site | Wild type | 20 ± 2 | 23 ± 0.7 |
Abasic site | K229L | 21 ± 0.7 | 21 ± 1.4 |
Abasic site | K229R | 21 ± 2 | 21 ± 0.7 |
No damage | Wild type | 2.5 ± 0.1 | 2.8 ± 0.4 |
No damage | K229L | 3.7 ± 0.2 | 3.8 ± 0.4 |
No damage | K229R | 7.6 ± 0.7 | 5.8 ± 1 |
Binding is expressed as the percentage of the input DNA retained on the filter. Binding efficiencies were tested in presence and absence of 1 mM MnCl2.