Table I.
Kinetic Rate Constants and Equilibrium Dissociation Constant of NOR Antibody Fragments as Determined by BIAcore. Purified NOR or NOR in Complex with VHHn Were Injected over the Sensor Chip Coated with the Corresponding VHHn Fragments
| kon105 M−1s−1 | koff 10−4s−1 | Kdiss 10−9M | |
|---|---|---|---|
| NOR+ | |||
| VHHn01 | 0.7 ± 0.2 | 6.2 ± 1.2 | 9.2 ± 1.1 |
| VHHn02 | 1.4 ± 0.3 | 6.7 ± 1.6 | 5.2 ± 1.7 |
| VHHn03 | 1.5 ± 0.3 | 1.8 ± 0.2 | 1.3 ± 0.25 |
| VHHn41 | 1.1 ± 0.35 | 5.6 ± 2.0 | 5.8 ± 1.6 |
| VHHn27 | 0.3 ± 0.1 | 15 ± 6 | 45 ± 5 |
| NOR-VHHn03+ | |||
| VHHn01 | nc | nc | nc |
| VHHn02 | nc | nc | nc |
| VHHn03 | nc | nc | nc |
| VHHn41 | 1.2 ± 0.4 | 8.3 ± 2.0 | 7.0 ± 1.5 |
| VHHn27 | 2.2 ± 0.1 | 22 ± 1.5 | 11 ± 1.4 |
| NOR-VHHn03-VHHn41+ | |||
| VHHn27 | 7 ± 2 | 39 ± 0.5 | 5.9 ± 1.3 |
nc: not calculable.